Magnetic Collection
MagC
We glue magnetic resin to an already polymerized resin-embedded sample
The magnetic resin contains:
remote control of floating ultrathin sections
retrieval of the serial section order after collection
ease of automated localization of sections after collection
magnetic nanoparticles
a fluorescent dye
fluorescent particles
Hundreds of sections are cut
with a standard ultramicrotome
and float freely at the water surface
of a diamond knife with a large water boat.
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Our MagDrive device automatically and magnetically
agglomerates the sections
until they land onto an immersed substrate inside the water bath.
After acquiring an overview
of the collected sections
our provided software semi-automatically
finds their location.
The numbering of the sections is not physically conserved during MagC
because sections float freely and randomly at the water surface.
Which section is number 48? Which section is number 412?
Our software automatically retrieves the section numbering
provided one of the following options:
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with some EM imagery of the sample in the sections.
It is convenient when the operator plans to acquire EM imagery in all sections.
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with brightfield LM imagery of the sections.
It is well suited for semi-thin sections.
​
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with fluorescent LM of the fluorescent beads
contained in the magnetic resin part of each section
Once you know the coordinates and numbering of the sections
you can then acquire 3D LM and EM imagery
of your regions of interest in your serial sections.
The yellow boxes show EM regions of interest.
The blue overlays show the magnetic resin.
3D EM of the wafer shown above
