We glue magnetic resin to an already polymerized resin-embedded sample
The magnetic resin contains:
remote control of floating ultrathin sections
retrieval of the serial section order after collection
ease of automated localization of sections after collection
a fluorescent dye
Hundreds of sections are cut with a standard ultramicrotome
and float freely at the water surface of a diamond knife with a large boat.
An overview of the collected sections is acquired and
their location is automatically found
using state of the art machine learning algorithms
directly available in our SectionFinder app.
The numbering of the sections is not physically conserved during MagC
because sections float freely and randomly at the water surface.
Which section is number 48? Which section is number 412?
Section numbering is done in two steps:
the fluorescent beads contained in the magnetic portion of each section are imaged with automated fluorescence microscopy
with advanced algorithms available in our SectionOrderer app we determine the numbering by solving a so-called traveling salesman problem
The numbering can also be retrieved using Electron Microscopy imagery of your sample.
Once you know the coordinates and numbering of the sections
you can then acquire 3D LM and EM imagery of
your regions of interest in your serial sections.
Regions of interest shown in yellow dashed boxes
3D EM of the wafer shown above